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Labs

Accelerated MCB 4934 and MCB 4034L Lab Courses

The University of Florida Online BS degree has three credits of lab requirements, one online, and two on campus.

  • MCB 3015c Online Lab Skills Boot Camp (online) (pre-req for MCB 4934/3020L)
  • MCB 4934 Microbiology Lab Boot Camp (in person accelerated immersion lab, 5 days long) (pre-req for MCB 4034L)
  • MCB 4034L Advanced Microbiology Lab (in person accelerated immersion lab, 5 days long)

MCB 3015c will be taught in 2018 during the spring semester as a fully online course.
MCB 4934 (3020L) will be taught in 2018 during the summer from May 5th to May 9th, EVERY DAY ALL DAY LONG (8-5)
MCB 4034L will be taught in 2018 during the summer from May 11th to May 15th, EVERY DAY ALL DAY LONG (8-5)

Let me know if you have any questions. moli@ufl.edu

Course Name Description Date
MCB4934 Microbiology Lab Immersion starts, Intron and lab safety, Mod 1 Microscopy, wet mount, phylogeny, Mod 2 Microbes in and around you & unknown project, Mod 3 Pipetting and graphing Saturday, May 5, 2018
MCB4934 Microbiology Lab Mod 4 Enumeration, Mod 5 Visualization and Gram stain, Mod 6 Biochemical tests, Gideon Sunday, May 6, 2018
MCB4934 Microbiology Lab Mod 7 gDNA extraction and PCR, Bioinformatics; Mod 8 pGLO transformation, Review for midterm Monday, May 7, 2018
MCB4934 Microbiology Lab Mod 9 Parasites, Mod 10 Water lab Mod 11 Start food lab – Midterm exam Tuesday, May 8, 2018
MCB4934 Microbiology Lab Mod 11 Food lab, Mod 7 Bioinformatics results – Field Trip Wednesday, May 9, 2018
MCB4034L

Advanced Microbiology Lab

Introduction to Course, pre-learning assessment, discuss ELNs, LAB 1: Screen Transposon mutant library for virulence phenotypes; sub-culturing for LABS 2-3, LAB 2: C. elegans culture Friday, May 11, 2018
MCB4034L

Advanced Microbiology Lab

LAB 3: Analysis of LAB 1 results, LAB 4: Isolate genomic DNA, LAB 5: Phage transduction of transposon mutant, **Go over Final presentation assignment** Saturday, May 12, 2018
MCB4034L

Advanced Microbiology Lab

LAB 6: Calculate DNA purity and concentration; Setup PCR check of genomic DNA, LAB 7: subculture wild-type and mutant GFP cultures for C. elegans infections (LAB 10), LAB 8: Analyze transduction results, **Go over abstract-writing assignment, REVIEW FOR MIDTERM EXAM** Sunday, May 13, 2018
MCB4034L

Advanced Microbiology Lab

Morning – MIDTERM EXAM (covers LABS 1-7 lectures and lab experiments), LAB 9: C. elegans infection part 1 (for RNA isolation), **Go over Bioinformatics assignment** Monday, May 14, 2018
MCB4034L

Advanced Microbiology Lab

LAB 10: C. elegans infection part 2 (for fluorescent microscopy), LAB 11: RNA isolations from C. elegans infection (setup in LAB 9), LAB 12: Determine RNA purity/concentration, cDNA synthesis
LAB 13: Fluorescence microscopy of C. elegans infection (setup in LAB 10), LAB 14: Agarose gel electrophoresis, LAB 15: Real-time PCR of cDNA to quantify gene expression, REVIEW SESSION (FINAL PRESENTATIONS DURING AFTERNOON PERIOD)
Tuesday, May 15, 2018